At the heart of QIAGEN’s business is a vision to make improvements in life possible.
We are on an exciting mission to make a real difference in science and healthcare. We are still the entrepreneurial company we started out as and have today achieved a size where we can bring our full power to many initiatives and to our presence across the globe.The Scientist will join the PCR and Enzymes R&D Team to develop and characterize innovative enzymes in the field of life sciences and biotechnology. The ideal candidate will have experience in protein engineering and expression. An expertise in designing and constructing expression vectors.
Strong experience in protein purification processes will be critical. The person will be working in a cross-functional team and required to support both internal and external (customer defined) projects. Duties include overexpression, screening, and biochemical characterization of novel enzymes and enzyme variants. Development of enzyme assay and/or applications (methods and workflow development) will also be required.
Responsibilities:
- Design and construct expression vectors.
- Perform protein expression and purification at different scales.
- Design and implement assays for enzyme characterization.
- Use rational design, structure analysis, directed evolution methods and protein engineering approaches to create novel enzymes or variants.
- Identify new opportunities and expand capabilities. Initiate and lead product development in developing innovative technologies and products.
- Ph.D. degree in Biochemistry, Molecular Biology, Enzymology or related field or MS with 3-5 year of Biotech industry experience.
- Extensive experience in protein expression in coli and purification techniques including ion exchange and affinity chromatography, dialysis, filtration, electrophoresis, and protein characterization and analytical methods such as thermal shift assay.
- Broad experience and knowledge in molecular biology techniques including PCR, gene assembly and cloning; protein engineering methods such as mutagenesis, fusion protein construction, and variant library generation methods.
- In-depth knowledge of enzymes used in molecular biology, especially polymerases and ligases, and molecular biology methods for nucleic acid analysis.
- Familiar with diverse software packages for sequence editing, primer design, sequence blasting, alignment, and molecular modeling software.
- Working experience with AKTA FPLC systems and knowledge of protein structural biology is a plus.
- Able to work in a fast-paced and collaborative team environment.
- Excellent analytical thinking, problem solving, timeline management and interpersonal skills.
- Strong organizational skills, and solid communication skills including oral, written and presentation techniques.
At the heart of QIAGEN are our people who drive our success. We act with passion, always challenging the status quo to drive innovation and continuous improvement. We inspire with our leadership and make an impact with our actions. We create a collaborative, safe and engaging workplace which forms the basis for high performing individuals and teams. We drive accountability and entrepreneurial decision-making and want you to excel your growth and shape the future of QIAGEN.
QIAGEN is committed to creating a diverse environment and is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex (including pregnancy, childbirth, and medical conditions related to pregnancy, childbirth, or breastfeeding), sex stereotyping (including but not limited to assumptions about a person’s appearance or behavior, gender roles, gender expression, or gender identity), gender, gender identity, gender expression, national origin, age, mental or physical disability, ancestry, medical condition, marital status, military or veteran status, citizenship status, sexual orientation, genetic information, or any other status protected by applicable law.QIAGEN complies with applicable laws regarding the confidentiality of employee health information and the reasonable accommodation of individuals with disabilities and/or sincerely held religious beliefs. Candidates who require accommodation should contact Benefits@QIAGEN.com . This policy is part of QIAGEN’s ongoing efforts to support public health efforts and to ensure the safety and-well-being of its employees, customers, business partners, and community.
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